You should have received a copy of the GNU General Public License along with this program. To keep the original images select the box next to Keep Source Images. You can give the stack a new name, or keep the generic ‘Stack’. It works with stacks, is parallelized, has a preview mode and is able to resolve overlapping lines. The different colours represent different points in time. I’ve trying to measure the areas that each coloured line encloses in the following image: These are traces of wounds on a patient made by hand and scanned in. IEEE Transactions on Pattern Analysis and Machine Intelligence, 20 (2), pp.113125. Hi, I’m super new to ImageJ/Fiji and image analysis in general. An unbiased detector of curvilinear structures. See the GNU General Public License for more details. To create a stack from multiple individual images go to Image -> Stacks -> Images to Stack. This plugin implements and extends the ridge / line detection algorithm described in: Steger, C., 1998. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. This program is free software you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation either version 3 of the License, or (at your option) any later version. Cordelières, A guided tour into subcellular colocalization analysis in light microscopy, Journal of Microscopy, Volume 224, Issue 3: 213-232 License The original plugin was published here: S. V2.0 plugin is now contained in Fiji just use Help › Update Fiji to get it. The plugin’s manual might be downloaded from ImageJ’s wiki. generates results representations such as:Īs ImageJ’s “Analyze Particles” function, 3D-OC also has a “redirect to” option, allowing one image to be taken as a mask to quantify intensity related parameters on a second image.
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